bone sialoprotein function

1,2 PP was identified in 1967 by Veis and Perry. To cite this version: Decreasing PP i by Ank ablation in Bsp-/-mice reestablished cementum but did … Bone sialoprotein (BSP) is a highly sulphated and glycosylated phosphoprotein that is a major constituent of bone and other mineralized connective tissues. Osf2, a member of the Cbf/runt family of transcription factors, is required for the development of osteoblasts in vivo and has been reported to stimulate the transcription of BSP when overexpressed in mesenchymal cell lines. Luc Malaval, Nd ey e Mari eme Wade-Gu eye, Maya Boudi a, Jia Fei, Ralph Zirngibl, Frieda Chen, Norbert Laroche, Jean-Paul Roux, Brigitte Burt-Pichat, Fran˘cois Duboeuf, et al. Probably important to cell-matrix interaction. and function and thereby pointing to the possibility that these SRLPs could be novel targets to modulate bone turnover. Bone sialoprotein (BSP) is a sulfated and phosphorylated glycoprotein found almost exclusively in mineralized connective tissues. Bone sialoprotein (BSP), an early phenotypic marker of osteoblast and cementoblast differentiation, has been implicated in the nucleation of hydroxyapatite during bone formation. Google Scholar Positive staining (arrows) is present in the nidus of cartilage for bone sialoprotein. Spider silk-bone sialoprotein fusion proteins for bone tissue engineering† S abılvia Gomes,abc Isabel B. Leonor,ab Joa˜o F. Mano, Rui L. Reis*ab and David L. Kaplan*c Received 9th January 2011, Accepted 14th March 2011 DOI: 10.1039/c1sm05024a The remarkable mechanical characteristics of the spider silk protein major ampullate spidroin protein Serum levels of bone sialoprotein, osteopontin, and beta2-microglobulin in stage I of multiple myeloma. Bone sialoprotein expression in primary human breast cancer is associated with bone metastases development. GeneRIFs: Gene References Into Functions. To test this hypothesis, the effects of BSP and osteopontin on hydroxyapatite crystal formation were determined by using a steady-state agarose gel system. [ Links ] 15. Appears to form an integral part of the mineralized matrix. In: Biology and chemistry of mineralized tissues. (A) To stimulate forward or reverse signaling, ephrin B1-Fc, ephrin B2-Fc, or IgG-Fc fragments were clustered with anti-Fc antibody at 2 µg/mL for 1 hr, and then primary mouse osteoblasts were cultured with 50 µg/mL ascorbic acid and 10 mM β-glycerophosphate for 17 days on the clustered proteins. One of the cDNA clones isolated from a rat osteosarcoma (ROS 17/2.8) phage lambda gt11 library had a 1473-base-pair-long insert that encoded a protein with 317 amino acid residues. Bone sialoprotein (BSP) and osteopontin, the major phosphorylated proteins of mammalian bone, have been proposed to function in the initiation of mineralization. Binds tightly to hydroxyapatite. Bone sialoprotein plays a functional role in bone formation and osteoclastogenesis. Study analyzed circulating bone sialoprotein (BSP) in a large cohort of patients with liver cirrhosis. Promotes Arg-Gly-Asp-dependent cell attachment. BSP, a bone … Although BSP can mediate cell attachment through an RGD sequence and binds selectively to hydroxyapatite, its precise function in mineralized tissues is unknown. This review describes normal bone anatomy and physiology as an introduction to the subsequent articles in this section that discuss clinical applications of iliac crest bone biopsy. glycoprotein of bone and is also expressed in a wide variety of other cells and tissues, including immune cells, skin, and blood vessels. Elucidating the functions of bone sialoprotein and OPN in bone formation. Expression of bone sialoprotein in primary human breast cancer is associated with poor survival. OPN, a multifunctional protein, is considered crucial for bone remodeling, biomineralization, and periodontal remodeling during mechanical tension and stress (orthodontic tooth movement). Tartrate-resistant acid phosphatase (TRAP or TRAPase), also called acid phosphatase 5, tartrate resistant (ACP5), is a glycosylated monomeric metalloprotein enzyme expressed in mammals. Disclosures: Y Bi, None. Bone sialoprotein (BSP) and osteopontin (OPN) belong to the small integrin‐binding ligand N‐linked glycoprotein (SIBLING) family, whose members interact with bone cells and bone mineral. Figure 4. BSP is a significant component of the bone extracellular matrix and has been suggested to constitute approximately 8% of all non-collagenous proteins found in bone … Theresultsshow thattheArg-Gly-Aspsequencealso conferscell-binding prop-erties on bone-specific sialoprotein. L. Bellahcene A, Menard S, Bufalino R, Moreau L & Castronovo V (1996). IBSP (Integrin Binding Sialoprotein) is a Protein Coding gene. Function. Bone sialoprotein (BSP) is a phosphorylated and sulfated glycoprotein that is a major noncollagenous protein of bone and other mineralizing connective tissues. Bone sialoprotein (BSP) is a component of mineralized tissues such as bone, dentin, cementum and calcified cartilage. bone sialoprotein 2, bone sialoprotein II, cell-binding sialoprotein. OPN knockout (−/−) mice do not lose bone in a model of hindlimb disuse (tail suspension), showing the importance of OPN in bone remodeling.We report that BSP −/− mice are viable and breed normally, but their weight and size are lower than wild-type (WT) mice. Wecould demonstrate that synthetic Arg-Gly-Asp-containing peptides efficiently inhibited the attach-mentofcellstosialoprotein-coatedsubstrates. NX_P21815 - IBSP - Bone sialoprotein 2 - Function. bone have been described, and some clues to their functions have been presented (3,4). Slavkin H, Price P, editors. Bone Sialoprotein (BSP-II) Based on chromatographic characteristics and compositional analysis, it is apparently a fragment of the sialoprotein (BSP-II) molecule that was initially described by Herring and coworkers68, 141 and later isolated in an intact form93,105 and found to have an apparent molecular weight of ~75,000 by SDS-PAGE. 4,5 DSP was identified in 1981. To determine the molecular mechanism of IGF-I regulation of osteogenesis, we analyzed the effects of IGF-I on the expression of BSP in osteoblast-like Saos2 and in rat stromal bone marrow (RBMC-D8) cells. Bone Sialoprotein (BSP) is a member of the "Small Integrin-Binding Ligand N-linked Glycoproteins" (SIBLING) extracellular matrix protein family of mineralized tissues. Addition of recombinant BSP altered Spp1, Ank, and Enpp1 mRNA expression in cementoblasts, in vitro. BSP has been less studied than other SIBLING proteins such as Osteopontin (OPN), which is coexpressed with it in several skeletal ce … Bone sialoprotein (BSP), is thought to function in the initial mineralization of bone, is selectively expressed by differentiated osteoblast. Journal of Bone and Mineral Research, 11: 665-670. bone sialoprotein 2, BSP II, bone sialoprotein II, cell-binding sialoprotein. Annexin A2 also known as annexin II is a protein that in humans is encoded by the ANXA2 gene.. Annexin 2 is involved in diverse cellular processes such as cell motility (especially that of the epithelial cells), linkage of membrane-associated protein complexes to the actin cytoskeleton, endocytosis, fibrinolysis, ion channel formation, and cell matrix interactions. 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